The most significant issues in the early phases of plant tissue culture are the browning of the explant and media. The objective of this research was to determine how well citric acid (CTR, 20 and 40 mg L-1), ascorbic acid (ASA, 200 and 400 mg L-1), and polyvinylpyrrolidone (PVP, 20 and 40 mg L-1) could help Phalaenopsis “Univivace” overcome medium browning during the stages of shoot multiplication (MS medium supplemented with 150 mL L-1 coconut water, 10 mg L-1 adenine sulfate, 1 mg L-1 thidiazuron, and 15 g L-1 sucrose) and rooting of shoots (MS medium with 25 mL L-1 coconut water, 1 mg L-1 indole butyric acid, 15 g L-1 sucrose). Numerous metrics were estimated, including the number of shoots that were regenerated during the shoot regeneration stage, the number of roots that were regenerated during the shoot rooting stage, and a number of growth parameters. The quantity of carotenoid and chlorophyll pigments, PSII quantum yield (Fv/Fm), the effective PSII quantum yield (YII), and the non-photochemical quenching (NPQ) were also measured. Additionally, oxidative stress enzyme malonaldehyde (MDA) and preventive antioxidant enzymes including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were measured in regenerated shoots and plantlets. The results showed that the medium browning issues during the shoot and root regeneration stages were resolved by supplementing with CTR, ASA, and PVP. In terms of shoot regeneration, rooting of shoots, and enhancing shoot and plantlet growth metrics, 40 mg L-1 CTR supplementation was determined to be superior overall.